NCMS Contractor Support
DOT Regulations - 49 CFR Part 40
Subpart F - Drug Testing Laboratories

§ 40.82 What drugs do laboratories test for?

December 18, 2025 17:21

As a laboratory, you must test for the following five drugs or classes of drugs in a DOT drug test. You must not test “DOT specimens” for any other drugs.

(a) Marijuana metabolites.

(b) Cocaine metabolites.

(d) Opioids.

(e) Phencyclidine (PCP).

[82 FR 52244, Nov. 13, 2017. Redesignated at 88 FR 27643, May 2, 2023]

This information was copied from a federal government resource. Always verify the accuracy of this information against the most recent version of the Code of Federal Regulations: https://www.ecfr.gov/current/title-49/subtitle-A/part-40 or the Office of Drug & Alcohol Policy & Compliance: https://www.transportation.gov/odapc.

Articles in this section

§ 40.81 What laboratories may be used for DOT drug testing?
§ 40.82 What drugs do laboratories test for?
§ 40.83 How do laboratories process incoming specimens?
Section 40.83 Questions and Answers
§ 40.84 How long does the laboratory retain specimens after testing?
§ 40.85 What are the cutoff concentrations for urine drug tests?
§ 40.86 What is urine validity testing, and are laboratories required to conduct it?
§ 40.87 What validity tests must laboratories conduct on primary urine specimens?
§ 40.88 What criteria do laboratories use to establish that a urine specimen is dilute or substituted?
§ 40.89 What are the adulterant cutoff concentrations for initial and confirmation urine tests?